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Western Blot analysis of HepG2 cells using VHR Polyclonal Antibody
Western Blot analysis of HepG2 cells using VHR Polyclonal Antibody
Western Blot analysis of HepG2 cells using VHR Polyclonal Antibody

DUSP3 Antibody

CSB-PA005173
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDUSP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    DUSP3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1845
  • Target name
    DUSP3
  • Target description
    dual specificity phosphatase 3
  • Target synonyms
    VHR, dual specificity protein phosphatase 3, dual specificity protein phosphatase VHR, serine/threonine specific protein phosphatase, vaccinia H1-related phosphatase, vaccinia virus phosphatase VH1-related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51452
  • Protein Name
    Dual specificity protein phosphatase 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of DUSP3 using anti-DUSP3 antibody (A06135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP3 antigen affinity purified polyclonal antibody (Catalog # A06135) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP3 at approximately 20 kDa. The expected band size for DUSP3 is at 20 kDa.
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