
Immunofluorescence analysis of methanol-fixed HeLa, using Dynamin 2(GTX113171) antibody at 1:500 dilution.
Dynamin 2 antibody [N1N3]
GTX113171
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDNM2
Overview
- SupplierGeneTex
- Product NameDynamin 2 antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.06 mg/ml
- ConjugateUnconjugated
- Gene ID1785
- Target nameDNM2
- Target descriptiondynamin 2
- Target synonymsCMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin-2, dynamin II
- HostRabbit
- IsotypeIgG
- Protein IDP50570
- Protein NameDynamin-2
- Scientific DescriptionDynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113171/GTX113171_40422_20170518_WB_R_w_23060500_200.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113171/GTX113171_44538_20220114_WB_w_23060500_328.webp)

![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (GTX113171) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113171/GTX113171_40422_20170608_WB_R_brain_w_23060501_391.webp)
![Dynamin 2 antibody [N1N3] detects Dynamin 2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Dynamin 2 antibody [N1N3] (GTX113171) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min Dynamin 2 antibody [N1N3] detects Dynamin 2 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Dynamin 2 antibody [N1N3] (GTX113171) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX113171/GTX113171_40422_IHC_w_23060501_922.webp)







![Dynamin 2 antibody [C2C3], C-term detects DNM2 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DNM2 protein stained by Dynamin 2 antibody [C2C3], C-term (GTX109652) diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]](https://www.genetex.com/upload/website/prouct_img/normal/GTX109652/GTX109652_40408_IFA_w_23060500_969.webp)