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Dynamin 2 Antibody (YA2464, Rabbit)

HY-P82719
MedChem Express
TargetDNM2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Dynamin 2 Antibody (YA2464, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1785
  • Target name
    DNM2
  • Target description
    dynamin 2
  • Target synonyms
    CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin-2, dynamin II
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Dynamin 2 Antibody (YA2464) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2464), targeting Dynamin 2, with a predicted molecular weight of 98 kDa (observed band size: 98 kDa). Dynamin 2 Antibody (YA2464) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 100 kDa. The expected band size for DNMT3B is at 96 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Dynamin 2 Polyclonal Antibody, Unconjugated (bs-0574R) at 1:400 overnight at 4°C, DAB staining.
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