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DYNLL1 Antibody (YA2724, Rabbit)

HY-P82979
MedChem Express
TargetDYNLL1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DYNLL1 Antibody (YA2724, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8655
  • Target name
    DYNLL1
  • Target description
    dynein light chain LC8-type 1
  • Target synonyms
    DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1, dynein light chain 1, cytoplasmic, 8 kDa dynein light chain, cytoplasmic dynein light polypeptide, dynein, cytoplasmic, light polypeptide 1, protein inhibitor of neuronal nitric oxide synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    DYNLL1 Antibody (YA2724) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2724), targeting DYNLL1, with a predicted molecular weight of 10 kDa (observed band size: 10 kDa). DYNLL1 Antibody (YA2724) can be used for WB, IHC-P, IP experiment in human, mouse background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (A03454-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: human Mcf-7 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYNLL1/PIN antigen affinity purified polyclonal antibody (Catalog # A03454-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYNLL1/PIN at approximately 12KD. The expected band size for DYNLL1/PIN is at 12KD.
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