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ProductsBack/DYNLL1 Recombinant Antbody Kit (KD-Validated)

DYNLL1 Recombinant Antbody Kit (KD-Validated)

BSM-90418R
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetDYNLL1
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Overview

  • Supplier
    Bioss
  • Product Name
    DYNLL1 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID8655
  • Target name
    DYNLL1
  • Target description
    dynein light chain LC8-type 1
  • Target synonyms
    DLC1, DLC8, DNCL1, DNCLC1, LC8, LC8a, PIN, hdlc1, dynein light chain 1, cytoplasmic, 8 kDa dynein light chain, cytoplasmic dynein light polypeptide, dynein, cytoplasmic, light polypeptide 1, protein inhibitor of neuronal nitric oxide synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63167
  • Protein Name
    Dynein light chain 1, cytoplasmic
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DYNLL1/PIN using anti-DYNLL1/PIN antibody (A03454-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: human Mcf-7 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYNLL1/PIN antigen affinity purified polyclonal antibody (Catalog # A03454-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYNLL1/PIN at approximately 12KD. The expected band size for DYNLL1/PIN is at 12KD.
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