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ProductsBack/E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]
Detection of endogenous E - cadherin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab 22F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB 231; lane 7: T47D
Detection of endogenous E - cadherin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab 22F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB 231; lane 7: T47D
Detection of endogenous E - cadherin Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab 22F8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB 231; lane 7: T47D

E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]

AM00010PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetCDH1
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    E Cadherin (CDH1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 22F8]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    22F8
  • Gene ID999
  • Target name
    CDH1
  • Target description
    cadherin 1
  • Target synonyms
    Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO, cadherin-1, CAM 120/80, E-cadherin 1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epididymis secretory sperm binding protein, epithelial cadherin, uvomorulin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12830
  • Protein Name
    Cadherin-1
  • Scientific Description
    E Cadherin (CDH1) (incl. pos. control) mouse monoclonal antibody, clone 22F8, Purified
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of E Cadherin using anti-E Cadherin antibody (PB9561). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E Cadherin antigen affinity purified polyclonal antibody (Catalog # PB9561) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E Cadherin at approximately 130 kDa. The expected band size for E Cadherin is at 97 kDa.
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