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ProductsBack/E2F6 Monoclonal Antibody

E2F6 Monoclonal Antibody

CAB11546
Assay Genie
TargetRAB7A
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    E2F6 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IP,ChIP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7879
  • Target name
    RAB7A
  • Target description
    RAB7A, member RAS oncogene family
  • Target synonyms
    CMT2B, PRO2706, RAB7, ras-related protein Rab-7a, RAB7, member RAS oncogene family, Ras-associated protein RAB7
  • Host
    Rabbit
  • Protein IDP51149
  • Protein Name
    Ras-related protein Rab-7a
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEL whole cell lysates. Lane 4: rat L6 whole cell lysates. Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7/RAB7A antigen affinity purified polyclonal antibody (Catalog # A02409-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7/RAB7A at approximately 23 kDa. The expected band size for RAB7/RAB7A is at 23 kDa.
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