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RAB7A / RAB7 Antibody

LS-C747860
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRAB7A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RAB7A / RAB7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 23kDa, while the observed MW by Western blot was 23kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 23kDa, while the observed MW by Western blot was 23kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7879
  • Target name
    RAB7A
  • Target description
    RAB7A, member RAS oncogene family
  • Target synonyms
    CMT2B, PRO2706, RAB7, ras-related protein Rab-7a, RAB7, member RAS oncogene family, Ras-associated protein RAB7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAB7 Polyclonal Antibody(bs-6703R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEL whole cell lysates. Lane 4: rat L6 whole cell lysates. Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7/RAB7A antigen affinity purified polyclonal antibody (Catalog # A02409-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7/RAB7A at approximately 23 kDa. The expected band size for RAB7/RAB7A is at 23 kDa.
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