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EAAT3 Antibody (YA2365, Rabbit)

HY-P82620
MedChem Express
TargetSLC1A1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    EAAT3 Antibody (YA2365, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6505
  • Target name
    SLC1A1
  • Target description
    solute carrier family 1 member 1
  • Target synonyms
    DCBXA; EAAC1; EAAT3; excitatory amino acid carrier 1; excitatory amino acid transporter 3; neuronal and epithelial glutamate transporter; SCZD18; sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    EAAT3 Antibody (YA2365) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2365), targeting EAAT3, with a predicted molecular weight of 57 kDa (observed band size: 70 kDa). EAAT3 Antibody (YA2365) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EAAT3/SLC1A1 using anti-EAAT3/SLC1A1 antibody (A02367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT3/SLC1A1 antigen affinity purified polyclonal antibody (Catalog # A02367-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT3/SLC1A1 at approximately 70KD. The expected band size for EAAT3/SLC1A1 is at 70KD.
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