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Western Blot analysis of 293 cells using EAAT3 Polyclonal Antibody
Western Blot analysis of 293 cells using EAAT3 Polyclonal Antibody
Western Blot analysis of 293 cells using EAAT3 Polyclonal Antibody

SLC1A1 Antibody

CSB-PA002241
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC1A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC1A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6505
  • Target name
    SLC1A1
  • Target description
    solute carrier family 1 member 1
  • Target synonyms
    DCBXA, EAAC1, EAAT3, SCZD18, hEAAC1, excitatory amino acid transporter 3, excitatory amino acid carrier 1, neuronal and epithelial glutamate transporter, sodium-dependent glutamate/aspartate transporter 3, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43005
  • Protein Name
    Excitatory amino acid transporter 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EAAT3/SLC1A1 using anti-EAAT3/SLC1A1 antibody (A02367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT3/SLC1A1 antigen affinity purified polyclonal antibody (Catalog # A02367-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT3/SLC1A1 at approximately 70KD. The expected band size for EAAT3/SLC1A1 is at 70KD.
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