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ProductsBack/EBI3 Rabbit Polyclonal Antibody
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-2: Mouse kidney tissue 293T cell lysates Primary antibody: TA365338 (EBI3 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 minutes
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-2: Mouse kidney tissue 293T cell lysates Primary antibody: TA365338 (EBI3 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 minutes
Gel: 12%SDS-PAGE Lysate: 40 microg Lane 1-2: Mouse kidney tissue 293T cell lysates Primary antibody: TA365338 (EBI3 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 minutes

EBI3 Rabbit Polyclonal Antibody

TA365338
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetEBI3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    EBI3 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10148
  • Target name
    EBI3
  • Target description
    Epstein-Barr virus induced 3
  • Target synonyms
    cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; IL27 subunit; IL-27 subunit beta; IL27B; IL-27B; IL35 subunit; IL35B; interleukin-27 subunit beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14213
  • Protein Name
    Interleukin-27 subunit beta
  • Scientific Description
    EBI3 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBI3 using anti-EBI3 antibody (M07190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBI3 antigen affinity purified monoclonal antibody (Catalog # M07190-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBI3 at approximately 30 kDa. The expected band size for EBI3 is at 25,30 kDa.
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