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Anti-human EBI3 mAb (MT23A14), unconjugated

Anti-human EBI3 mAb (MT23A14), unconjugated

3459-3
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetEBI3
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-human EBI3 mAb (MT23A14), unconjugated
  • Delivery Days Customer
    5
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT23A14
  • Conjugate
    Unconjugated
  • Gene ID10148
  • Target name
    EBI3
  • Target description
    Epstein-Barr virus induced 3
  • Target synonyms
    IL-27B, IL27B, IL35B, interleukin-27 subunit beta, EBV-induced gene 3 protein, Epstein-Barr virus induced gene 3, IL-27 subunit beta, IL27 subunit, IL35 subunit, cytokine receptor, epstein-Barr virus-induced gene 3 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of the EBI3 subunit of human IL-27 and IL-35 in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBI3 using anti-EBI3 antibody (M07190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBI3 antigen affinity purified monoclonal antibody (Catalog # M07190-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBI3 at approximately 30 kDa. The expected band size for EBI3 is at 25,30 kDa.
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