ICC/IF analysis of HDFn cells using GTX54746 ECH1 antibody [9D11AF3]. Green : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde 20 mins Permeabilization : Triton X-100 (0.1%, 15 min) Dilution : 4 microg/ml overnight at 4oC
ICC/IF analysis of HDFn cells using GTX54746 ECH1 antibody [9D11AF3]. Green : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde 20 mins Permeabilization : Triton X-100 (0.1%, 15 min) Dilution : 4 microg/ml overnight at 4oC
ECH1 antibody [9D11AF3]
GTX54746
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetECH1
Overview
- SupplierGeneTex
- Product NameECH1 antibody [9D11AF3]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9D11AF3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1891
- Target nameECH1
- Target descriptionenoyl-CoA hydratase 1
- Target synonymsdelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; delta3,5-delta2,4-dienoyl-coenzyme A isomerase; dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; enoyl-CoA hydratase 1, peroxisomal; epididymis secretory sperm binding protein; HPXEL; peroxisomal enoyl-CoA hydratase 1
- HostMouse
- IsotypeIgG1
- Protein IDQ13011
- Protein NameDelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
- Scientific DescriptionThis gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Rat
- Storage Instruction2°C to 8°C
- UNSPSC12352203