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ProductsBack/ECHS1 antibody [N1C2]

ECHS1 antibody [N1C2]

GTX114376
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetECHS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ECHS1 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1892
  • Target name
    ECHS1
  • Target description
    enoyl-CoA hydratase, short chain 1
  • Target synonyms
    ECHS1D, SCEH, mECH, mECH1, enoyl-CoA hydratase, mitochondrial, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial, enoyl-CoA hydratase, short chain, 1, mitochondrial, epididymis secretory sperm binding protein, short chain enoyl-CoA hydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30084
  • Protein Name
    Enoyl-CoA hydratase, mitochondrial
  • Scientific Description
    The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Haack TB, Jackson CB, Murayama K, et al. Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement. Ann Clin Transl Neurol. 2015,2(5):492-509. doi: 10.1002/acn3.189
    Read this paper

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Figure 1. Western blot analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody (Catalog # A04508-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECHS1 at approximately 29 kDa. The expected band size for ECHS1 is at 29 kDa.
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