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ECI1 / DCI Antibody (N-Terminus)

LS-C81259
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetECI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ECI1 / DCI Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1632
  • Target name
    ECI1
  • Target description
    enoyl-CoA delta isomerase 1
  • Target synonyms
    3,2 trans-enoyl-Coenzyme A isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; acetylene-allene isomerase; D3,D2-enoyl-CoA isomerase; DCI; delta(3),Delta(2)-enoyl-CoA isomerase; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); enoyl-CoA delta isomerase 1, mitochondrial; epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DCI using anti-DCI antibody (PA1763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody (Catalog # PA1763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.
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