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EDG3 Antibody (YA2359)

HY-P82614
MedChem Express
TargetS1PR3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    EDG3 Antibody (YA2359)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1903
  • Target name
    S1PR3
  • Target description
    sphingosine-1-phosphate receptor 3
  • Target synonyms
    bA791O21.3; C9orf108; C9orf47; EDG3; EDG-3; endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; sphingosine 1-phosphate receptor 3; sphingosine 1-phosphate receptor Edg-3; uncharacterized protein C9orf47
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    EDG3 Antibody (YA2359) is a non-conjugated IgG antibody, targeting EDG3, with a predicted molecular weight of 42 kDa (observed band size: 42 kDa). EDG3 Antibody (YA2359) can be used for WB experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of EDG3 using anti-EDG3 antibody (M03755-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG3 antigen affinity purified monoclonal antibody (Catalog # M03755-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG3 at approximately 42 kDa. The expected band size for EDG3 is at 42 kDa.
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