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Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: EDNRB antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 49, 60 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: EDNRB antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 49, 60 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse skeletal muscle tissue All lanes: EDNRB antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 49, 60 kDa Observed band size: 50 kDa

EDNRB Antibody

CSB-PA007404LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEDNRB
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Overview

  • Supplier
    Cusabio
  • Product Name
    EDNRB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1910
  • Target name
    EDNRB
  • Target description
    endothelin receptor type B
  • Target synonyms
    ABCDS; endothelin receptor non-selective type; endothelin receptor subtype B1; endothelin receptor type B; ETB; ET-B; ETB1; ETBR; ET-BR; ETRB; Hirschsprung disease 2; HSCR; HSCR2; WS4A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24530
  • Protein Name
    Endothelin receptor type B
  • Scientific Description
    Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDNRB using anti-EDNRB antibody (A01041). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDNRB antigen affinity purified polyclonal antibody (Catalog # A01041) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDNRB at approximately 45 kDa. The expected band size for EDNRB is at 49,59 kDa.
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Lane 1: Rat heart lysates; Lane 2: Mouse heart lysates probed with ETBR/Endothelin B Receptor Polyclonal Antibody, unconjugated (bs-4198R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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