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ProductsBack/EDNRB / Endothelin B Receptor Antibody

EDNRB / Endothelin B Receptor Antibody

LS-C335279
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetEDNRB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EDNRB / Endothelin B Receptor Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 48kDa/49kDa/59kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 48kDa/49kDa/59kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1910
  • Target name
    EDNRB
  • Target description
    endothelin receptor type B
  • Target synonyms
    ABCDS, ET-B, ET-BR, ETB, ETB1, ETBR, ETRB, HSCR, HSCR2, WS4A, endothelin receptor type B, Hirschsprung disease 2, endothelin receptor non-selective type, endothelin receptor subtype B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EDNRB using anti-EDNRB antibody (A01041). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDNRB antigen affinity purified polyclonal antibody (Catalog # A01041) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDNRB at approximately 45 kDa. The expected band size for EDNRB is at 49,59 kDa.
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