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EEA1 antibody

GTX82138
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetEEA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EEA1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. ICC/IF: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8411
  • Target name
    EEA1
  • Target description
    early endosome antigen 1
  • Target synonyms
    MST105, MSTP105, ZFYVE2, early endosome antigen 1, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2
  • Host
    Chicken
  • Isotype
    IgY
  • Protein IDQ15075
  • Protein Name
    Early endosome antigen 1
  • Scientific Description
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu JL, Kuan II, Guo JY, et al. SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron. iScience. 2023,26(2):105995. doi: 10.1016/j.isci.2023.105995
    Read this paper
  • Beaulieu A, Gravel É, Cloutier A, et al. Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium. J Virol. 2013,87(8):4237-51. doi: 10.1128/JVI.03005-12
    Read this paper
  • Smith JL, McBride CM, Nataraj PS, et al. Trafficking-deficient hERG K⁺ channels linked to long QT syndrome are regulated by a microtubule-dependent quality control compartment in the ER. Am J Physiol Cell Physiol. 2011,301(1):C75-85. doi: 10.1152/ajpcell.00494.2010
    Read this paper

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Figure 1. Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: humna K562 whole cell lysates, Lane 6: humna MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (Catalog # A02296-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
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