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ProductsBack/EEA1 antibody [C3], C-term

EEA1 antibody [C3], C-term

GTX109638
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEEA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EEA1 antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8411
  • Target name
    EEA1
  • Target description
    early endosome antigen 1
  • Target synonyms
    MST105, MSTP105, ZFYVE2, early endosome antigen 1, early endosome antigen 1, 162kD, early endosome-associated protein, endosome-associated protein p162, zinc finger FYVE domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15075
  • Protein Name
    Early endosome antigen 1
  • Scientific Description
    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cinato M, Guitou L, Saidi A, et al. Apilimod alters TGFβ signaling pathway and prevents cardiac fibrotic remodeling. Theranostics. 2021,11(13):6491-6506. doi: 10.7150/thno.55821
    Read this paper
  • Dey AK, Gonon A, Pécheur EI, et al. Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic Cells. Cells. 2021,10(1). doi: 10.3390/cells10010096
    Read this paper
  • Liang W, Moyzis AG, Lampert MA, et al. Aging is associated with a decline in Atg9b-mediated autophagosome formation and appearance of enlarged mitochondria in the heart. Aging Cell. 2020,19(8):e13187. doi: 10.1111/acel.13187
    Read this paper
  • Woodall BP, Orogo AM, Najor RH, et al. Parkin does not prevent accelerated cardiac aging in mitochondrial DNA mutator mice. JCI Insight. 2019,5(10):pii: 127713. doi: 10.1172/jci.insight.127713.
    Read this paper
  • Lai JH, Wang MY, Huang CY, et al. Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells. EMBO Rep. 2018,19(8). doi: 10.15252/embr.201846182
    Read this paper
  • Gopalakrishna R, Gundimeda U, Zhou S, et al. Laminin-1 induces endocytosis of 67KDa laminin receptor and protects Neuroscreen-1 cells against death induced by serum withdrawal. Biochem Biophys Res Commun. 2018,495(1):230-237. doi: 10.1016/j.bbrc.2017.11.015
    Read this paper
  • Chen NC, Chyau CC, Lee YJ, et al. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells. Sci Rep. 2016,6:20417. doi: 10.1038/srep20417
    Read this paper
  • Chou SF, Tsai ML, Huang JY, et al. The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion. PLoS Pathog. 2015,11(10):e1005123. doi: 10.1371/journal.ppat.1005123
    Read this paper
  • Fang CY, Chen SJ, Wu HN, et al. Honokiol, a Lignan Biphenol Derived from the Magnolia Tree, Inhibits Dengue Virus Type 2 Infection. Viruses. 2015,7(9):4894-910. doi: 10.3390/v7092852
    Read this paper
  • Luo YH, Wu SB, Wei YH, et al. Cadmium-based quantum dot induced autophagy formation for cell survival via oxidative stress. Chem Res Toxicol. 2013,26(5):662-73. doi: 10.1021/tx300455k
    Read this paper

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Figure 1. Western blot analysis of EEA1 using anti-EEA1 antibody (A02296-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: humna K562 whole cell lysates, Lane 6: humna MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 antigen affinity purified polyclonal antibody (Catalog # A02296-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEA1 at approximately 162 kDa. The expected band size for EEA1 is at 162 kDa.
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