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Western blot All lanes: Polycomb protein EED antibody at 4ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 54, 46 kDa Observed band size: 51 kDa
Western blot All lanes: Polycomb protein EED antibody at 4ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 54, 46 kDa Observed band size: 51 kDa
Western blot All lanes: Polycomb protein EED antibody at 4ug/ml Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 54, 46 kDa Observed band size: 51 kDa

EED Antibody

CSB-PA007408ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEED
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Overview

  • Supplier
    Cusabio
  • Product Name
    EED Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8726
  • Target name
    EED
  • Target description
    embryonic ectoderm development
  • Target synonyms
    COGIS; embryonic ectoderm development protein; HEED; polycomb protein EED; WAIT1; WD protein associating with integrin cytoplasmic tails 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75530
  • Protein Name
    Polycomb protein EED
  • Scientific Description
    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates Lys-9 and Lys-27 of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes Lys-26 trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 Lys-27, whereas H3 Lys-27 recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EED using anti-EED antibody (A01345-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (Catalog # A01345-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EED at approximately 70 kDa. The expected band size for EED is at 50 kDa.
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