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WB analysis of HepG2 (1), HeLa (2), HEK293 (3) and A431 (4) cell lysate using GTX60421 eEF2 antibody [5B6].
WB analysis of HepG2 (1), HeLa (2), HEK293 (3) and A431 (4) cell lysate using GTX60421 eEF2 antibody [5B6].
WB analysis of HepG2 (1), HeLa (2), HEK293 (3) and A431 (4) cell lysate using GTX60421 eEF2 antibody [5B6].

eEF2 antibody [5B6]

GTX60421
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetEEF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    eEF2 antibody [5B6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B6
  • Conjugate
    Unconjugated
  • Gene ID1938
  • Target name
    EEF2
  • Target description
    eukaryotic translation elongation factor 2
  • Target synonyms
    EEF-2, EF-2, EF2, SCA26, elongation factor 2, epididymis secretory sperm binding protein, polypeptidyl-tRNA translocase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13639
  • Protein Name
    Elongation factor 2
  • Scientific Description
    This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EEF2 using anti-EEF2 antibody (A00830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: zebrafish tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat pancrease tissue lysates, Lane 11: rat C6 whole cell lysates, Lane 12: mouse pancrease tissue lysates, Lane 13: mouse 3T3L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF2 antigen affinity purified polyclonal antibody (Catalog # A00830-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EEF2 at approximately 95 kDa. The expected band size for EEF2 is at 95 kDa.
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