EGF Antibody (clone R001, Preservative Free)

LifeSpan BioSciences

EGF Antibody (clone R001, Preservative Free)

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Block: In a functional ELISA, 1. 2 microg/ml of this antibody will block >80% of the binding of 5 microg/ml of biotinylated Human EGF to immobilized Recombinant Human EGFR coated at 10 microg/ml (100 microL/well) in the plate. Neutralization: The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2. 5-7. 5 ug/ml in the presence of 2 ng/mL Recombinant Human EGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.. Block (5 µg/ml), ELISA (1.2 µg/ml), Neut (2 ng/ml) Block: In a functional ELISA, 1. 2 µg/ml of this antibody will block >80% of the binding of 5 µg/ml of biotinylated Human EGF to immobilized Recombinant Human EGFR coated at 10 µg/ml (100 µL/well) in the plate. Neutralization: The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2. 5-7. 5 ug/ml in the presence of 2 ng/mL Recombinant Human EGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

R001

Unconjugated

...

EGF

epidermal growth factor

HOMG4, URG, pro-epidermal growth factor, beta-urogastrone

Rabbit

IgG

-20°C or -80°C,2°C to 8°C

12352203