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ProductsBack/EGF Rabbit Polyclonal Antibody
Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF antibody (Cat.-No.: AP20662PU-N)
Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF antibody (Cat.-No.: AP20662PU-N)
Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF antibody (Cat.-No.: AP20662PU-N)

EGF Rabbit Polyclonal Antibody

AP20662PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEGF
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    EGF Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1950
  • Target name
    EGF
  • Target description
    epidermal growth factor
  • Target synonyms
    HOMG4, URG, pro-epidermal growth factor, beta-urogastrone
  • Host
    Rabbit
  • Protein IDP01133
  • Protein Name
    Pro-epidermal growth factor
  • Scientific Description
    EGF rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EGF Polyclonal Antibody(bs-2008R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of EGF using anti-EGF antibody (PB9861). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # PB9861) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 134 kDa. The expected band size for EGF is at 133 kDa.
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