Detection of endogenous EGFR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-13G8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Detection of endogenous EGFR: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-13G8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
EGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]
AM00047PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetEGFR
Overview
- SupplierOriGene
- Product NameEGFR (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13G8]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID13G8
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostMouse
- IsotypeIgG1
- Scientific DescriptionEGFR (C-term) (incl. pos. control) mouse monoclonal antibody, clone 13G8, Purified
- ReactivityHuman, Mouse
- UNSPSC12352203