Antibody sensitivity Whole cell lysates of A431cells containing defined cell numbers per lane were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-20E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
Antibody sensitivity Whole cell lysates of A431cells containing defined cell numbers per lane were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-20E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells
EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]
AM00046PU-N
Overview
- SupplierOriGene
- Product NameEGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID20E12
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostMouse
- IsotypeIgG
- Protein IDP00533
- Protein NameEpidermal growth factor receptor
- Scientific DescriptionEGFR (Extracell. Dom.) (incl. pos. control) mouse monoclonal antibody, clone 20E12, Purified
- ReactivityHuman
- UNSPSC12352203