EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-11C2 (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control; Lane 2: 5 min EGF; Lane 3: 15 min EGF; Lane 4: 30 min EGF; Lane 5: 1h EGF; Lane 6; 2h EGF; Lane 7: 4h EGF; Lane 8: 8h EGF.
EGFR Activation Serum starved MDA-MB 468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab EGFR-11C2 (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control; Lane 2: 5 min EGF; Lane 3: 15 min EGF; Lane 4: 30 min EGF; Lane 5: 1h EGF; Lane 6; 2h EGF; Lane 7: 4h EGF; Lane 8: 8h EGF.
EGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]
AM00034PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetEGFR
Overview
- SupplierOriGene
- Product NameEGFR pTyr1069 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C2]
- Delivery Days Customer14
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID11C2
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostMouse
- IsotypeIgG1
- Protein IDP00533
- Protein NameEpidermal growth factor receptor
- Scientific DescriptionEGFR pTyr1069 (incl. pos. control) mouse monoclonal antibody, clone 11C2, Purified
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203