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ProductsBack/EGFR pTyr1172 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10G12]
Detection of endogenous EGFR Whole cell lysates of EGF-stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00041PU-N (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Detection of endogenous EGFR Whole cell lysates of EGF-stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00041PU-N (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2
Detection of endogenous EGFR Whole cell lysates of EGF-stimulated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with AM00041PU-N (0.5 ug/ ml) for 1 h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2

EGFR pTyr1172 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10G12]

AM00041PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetEGFR
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    EGFR pTyr1172 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10G12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10G12
  • Gene ID1956
  • Target name
    EGFR
  • Target description
    epidermal growth factor receptor
  • Target synonyms
    ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA, epidermal growth factor receptor, EGFR vIII, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor tyrosine kinase domain, erb-b2 receptor tyrosine kinase 1, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP00533
  • Protein Name
    Epidermal growth factor receptor
  • Scientific Description
    EGFR pTyr1172 (incl. pos. control) mouse monoclonal antibody, clone 10G12, Purified
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (A00023-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # A00023-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 180KD. The expected band size for EGFR is at 180KD.
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