Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-12A3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-12A3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
EGFR pTyr869 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12A3]
AM00031PU-N
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetEGFR
Overview
- SupplierOriGene
- Product NameEGFR pTyr869 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12A3]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID12A3
- Gene ID1956
- Target nameEGFR
- Target descriptionepidermal growth factor receptor
- Target synonymsavian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor; epidermal growth factor receptor tyrosine kinase domain; ERBB; ERBB1; erb-b2 receptor tyrosine kinase 1; ERRP; HER1; mENA; NISBD2; PIG61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
- HostMouse
- IsotypeIgG1
- Scientific DescriptionEGFR pTyr869 (incl. pos. control) mouse monoclonal antibody, clone 12A3, Purified
- ReactivityHuman, Mouse
- UNSPSC12352203