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ProductsBack/EGFR-pY1086 Polyclonal Antibody AE00216
Western Blot of 30ug lysate from serum-starved A431 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY1086 Rabbit Polyclonal Antibody AE00216 at 1ug/ml (1h at ambient temp). ECL staining by HRP. ECL staining by HRP
Western Blot of 30ug lysate from serum-starved A431 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY1086 Rabbit Polyclonal Antibody AE00216 at 1ug/ml (1h at ambient temp). ECL staining by HRP. ECL staining by HRP
Western Blot of 30ug lysate from serum-starved A431 with or without stimulation by 100mg/ml EGF (30 min, 37C) ), stained with EGFR-pY1086 Rabbit Polyclonal Antibody AE00216 at 1ug/ml (1h at ambient temp). ECL staining by HRP. ECL staining by HRP

EGFR-pY1086 Polyclonal Antibody AE00216

AE00216
Aeonian Biotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetEGFR

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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    EGFR-pY1086 Polyclonal Antibody AE00216
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1956
  • Target name
    EGFR
  • Target description
    epidermal growth factor receptor
  • Target synonyms
    ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA, epidermal growth factor receptor, EGFR vIII, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor tyrosine kinase domain, erb-b2 receptor tyrosine kinase 1, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00533
  • Protein Name
    Epidermal growth factor receptor
  • Scientific Description
    EGFR-pY1086 Polyclonal Antibody AE00216
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (A00023-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # A00023-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 180KD. The expected band size for EGFR is at 180KD.
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