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EHMT2 Antibody (YA2373)

HY-P82628
MedChem Express
TargetEHMT2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    EHMT2 Antibody (YA2373)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10919
  • Target name
    EHMT2
  • Target description
    euchromatic histone lysine methyltransferase 2
  • Target synonyms
    ankyrin repeat-containing protein; BAT8; C6orf30; euchromatic histone-lysine N-methyltransferase 2; G9A; G9A histone methyltransferase; GAT8; H3-K9-HMTase 3; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase EHMT2; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; KMT1C; lysine N-methyltransferase 1C; NG36
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    EHMT2 Antibody (YA2373) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2373), targeting EHMT2, with a predicted molecular weight of 132 kDa (observed band size: 170 kDa). EHMT2 Antibody (YA2373) can be used for WB, IHC-P experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EHMT2/G9A using anti-EHMT2/G9A antibody (A01055-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHMT2/G9A antigen affinity purified polyclonal antibody (Catalog # A01055-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHMT2/G9A at approximately 160-180 kDa. The expected band size for EHMT2/G9A is at 160-180 kDa.
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