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ProductsBack/EHMT2 / G9A Antibody

EHMT2 / G9A Antibody

LS-C331386
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEHMT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EHMT2 / G9A Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 19kDa/129kDa/132kDa, while the observed MW by Western blot was 132kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 19kDa/129kDa/132kDa, while the observed MW by Western blot was 132kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10919
  • Target name
    EHMT2
  • Target description
    euchromatic histone lysine methyltransferase 2
  • Target synonyms
    BAT8, C6orf30, G9A, GAT8, KMT1C, NG36, histone-lysine N-methyltransferase EHMT2, G9A histone methyltransferase, H3-K9-HMTase 3, HLA-B associated transcript 8, ankyrin repeat-containing protein, euchromatic histone-lysine N-methyltransferase 2, histone H3-K9 methyltransferase 3, histone-lysine N-methyltransferase, H3 lysine-9 specific 3, lysine N-methyltransferase 1C
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EHMT2/G9A using anti-EHMT2/G9A antibody (A01055-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHMT2/G9A antigen affinity purified polyclonal antibody (Catalog # A01055-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHMT2/G9A at approximately 160-180 kDa. The expected band size for EHMT2/G9A is at 160-180 kDa.
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