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EIF2 beta antibody

GTX106484
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEIF2S2
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Overview

  • Supplier
    GeneTex
  • Product Name
    EIF2 beta antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8894
  • Target name
    EIF2S2
  • Target description
    eukaryotic translation initiation factor 2 subunit beta
  • Target synonyms
    EIF2; EIF2B; EIF2beta; eIF-2-beta; eukaryotic translation initiation factor 2 subunit 2; eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa; PPP1R67; protein phosphatase 1, regulatory subunit 67
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20042
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 2
  • Scientific Description
    Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits, alpha, beta, and gamma, with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, which recycles the EIF-2 complex for another round of initiation. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Resources for the Comprehensive Discovery of Functional RNA Elements. Sundararaman B et al., 2016 Mar 17, Mol Cell
    Read more
  • Nuclear accumulation of mRNAs underlies G4C2-repeat-induced translational repression in a cellular model of C9orf72 ALS. Rossi S et al., 2015 May 1, J Cell Sci
    Read more
  • Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation. Knight JR et al., 2015 Jan 15, Biochem J
    Read more

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Figure 1. Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S2 antigen affinity purified polyclonal antibody (Catalog # PA2157) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S2 at approximately 38 kDa. The expected band size for EIF2S2 is at 38 kDa.
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