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Western Blot analysis of various cells using EIF2AK1 Polyclonal Antibody
Western Blot analysis of various cells using EIF2AK1 Polyclonal Antibody
Western Blot analysis of various cells using EIF2AK1 Polyclonal Antibody

EIF2AK1 Antibody

CSB-PA002969
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEIF2AK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF2AK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27102
  • Target name
    EIF2AK1
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 1
  • Target synonyms
    eukaryotic translation initiation factor 2-alpha kinase 1; HCR; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; heme-controlled repressor; heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; heme-regulated inhibitor; heme-regulated repressor; hemin-sensitive initiation factor 2-alpha kinase; hHRI; HRI; LEMSPAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQI3
  • Protein Name
    Eukaryotic translation initiation factor 2-alpha kinase 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK1 antigen affinity purified polyclonal antibody (Catalog # A05465-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2AK1 at approximately 71 kDa. The expected band size for EIF2AK1 is at 71 kDa.
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