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ProductsBack/EIF2AK2 / PKR Antibody (phospho-Thr451)

EIF2AK2 / PKR Antibody (phospho-Thr451)

LS-C17287
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEIF2AK2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EIF2AK2 / PKR Antibody (phospho-Thr451)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Positive control: HeLa cells stimulated with interferon gamma (IFN ) and calyculin.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (1:1000) Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Positive control: HeLa cells stimulated with interferon gamma (IFN ) and calyculin.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5610
  • Target name
    EIF2AK2
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 2
  • Target synonyms
    double stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; LEUDEN; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; PRKR; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.
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