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Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-EIF2AK2/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-EIF2AK2/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-EIF2AK2/PKR Polyclonal Antibody, Unconjugated (bs-1493R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

EIF2AK2 (PKR) Polyclonal Antibody

BS-1493R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEIF2AK2
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Overview

  • Supplier
    Bioss
  • Product Name
    EIF2AK2 (PKR) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5610
  • Target name
    EIF2AK2
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 2
  • Target synonyms
    DYT33, EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR, interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A protein kinase, double stranded RNA activated protein kinase, eIF-2A protein kinase 2, interferon-inducible elF2alpha kinase, p68 kinase, protein kinase R, protein kinase RNA-regulated, protein kinase, interferon-inducible double stranded RNA dependent, protein phosphatase 1, regulatory subunit 83, tyrosine-protein kinase EIF2AK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19525
  • Protein Name
    Interferon-induced, double-stranded RNA-activated protein kinase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Lopušná K, Benkóczka T, Lupták J, et al. Murine gammaherpesvirus targets type I IFN receptor but not type III IFN receptor early in infection. Cytokine. 2016,83:158-170. doi: 10.1016/j.cyto.2016.04.013
    Read this paper

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Figure 1. Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.
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