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Western Blot analysis of 3T3 cells using eIF2alpha Polyclonal Antibody
Western Blot analysis of 3T3 cells using eIF2alpha Polyclonal Antibody
Western Blot analysis of 3T3 cells using eIF2alpha Polyclonal Antibody

EIF2S1 Antibody

CSB-PA002295
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetEIF2S1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF2S1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1965
  • Target name
    EIF2S1
  • Target description
    eukaryotic translation initiation factor 2 subunit alpha
  • Target synonyms
    EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05198
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 1
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (P04387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified monoclonal antibody (Catalog # P04387) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 36 kDa. The expected band size for EIF2S1 is at 36 kDa.
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