More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/phospho-eIF4E (Ser209) Recombinant Antibody
Lane 1: Mouse spleen; Lane 2: Rat spleen cell lysate probed with eIF4E(S209) (2B1) Monoclonal Antibody (bsm-52152R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Mouse spleen; Lane 2: Rat spleen cell lysate probed with eIF4E(S209) (2B1) Monoclonal Antibody (bsm-52152R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: Mouse spleen; Lane 2: Rat spleen cell lysate probed with eIF4E(S209) (2B1) Monoclonal Antibody (bsm-52152R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

phospho-eIF4E (Ser209) Recombinant Antibody

BSM-52152R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEIF4E
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    EIF4E Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1977
  • Target name
    EIF4E
  • Target description
    eukaryotic translation initiation factor 4E
  • Target synonyms
    AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1, mRNA cap-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06730
  • Protein Name
    Eukaryotic translation initiation factor 4E
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Phospho-Eif4E (S209) Recombinant AntibodyCAC12446
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetEIF4E
  • SizePrice
Product group Antibodies
References
GeneTex
eIF4E antibodyGTX132092
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetEIF4E
  • SizePrice
Western Blot analysis of COLO205 cells using eIF4E Polyclonal Antibody
Product group Antibodies
Cusabio
EIF4E AntibodyCSB-PA002308
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetEIF4E
  • SizePrice
Product group Antibodies
MedChem Express
eIF4E Antibody (YA463, Rabbit)HY-P80118
TargetEIF4E
  • SizePrice
Product group Antibodies
Abbexa
EIF4E Antibody (HRP)ABX314116
ApplicationsELISA
  • SizePrice
Anti-eIF4E Antibody
Product group Antibodies
Antibodies.com
Anti-eIF4E AntibodyA35900
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-eIF4E Antibody, Affinity PurifiedA301-153A
ApplicationsImmunoPrecipitation, Western Blot
TargetEIF4E
  • SizePrice
Product group Antibodies
RayBiotech
Anti-EIF4E Antibody144-60024
ApplicationsImmunoFluorescence, Western Blot
TargetEIF4E
  • SizePrice
Product group Antibodies
Assay Genie
eIF4E AntibodyCAB0468
TargetEIF4E
  • SizePrice
Figure 1. Western blot analysis of EIF4E using anti-EIF4E antibody (M00135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse HEPA1-2 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-EIF4E Monoclonal AntibodyM00135
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetEIF4E
  • SizePrice