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Western Blot analysis of Jurkat cells using Hel-N1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Hel-N1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Hel-N1 Polyclonal Antibody

ELAVL2 Antibody

CSB-PA002900
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetELAVL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ELAVL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1993
  • Target name
    ELAVL2
  • Target description
    ELAV like RNA binding protein 2
  • Target synonyms
    HEL-N1, HELN1, HUB, ELAV-like protein 2, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B), ELAV like neuron-specific RNA binding protein 2, hu-antigen B, nervous system-specific RNA-binding protein Hel-N1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12926
  • Protein Name
    ELAV-like protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ELAVL2 HuB using anti-ELAVL2 HuB antibody (A06194-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysate, Lane 2: human A549 whole cell lysate, Lane 3: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL2 HuB antigen affinity purified polyclonal antibody (Catalog # A06194-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL2 HuB at approximately 45KD. The expected band size for ELAVL2 HuB is at 40KD.
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ReactivityHuman, Mouse, Rat
TargetELAVL2
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TargetELAVL2
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Product group Antibodies
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ReactivityMouse
TargetELAVL2
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TargetELAVL2
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Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA063001 antibody. Corresponding ELAVL2 RNA-seq data are presented for the same tissues.
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ELAVL2 antibody [HL1978] detects ELAVL2 protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ELAVL2 stained by ELAVL2 antibody [HL1978] (GTX637871) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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ELAVL2 antibody [HL1978]GTX637871
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TargetELAVL2
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