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Western blot All lanes: ELAVL4 antibody at 2microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 41, 43 kDa Observed band size: 42 kDa
Western blot All lanes: ELAVL4 antibody at 2microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 41, 43 kDa Observed band size: 42 kDa
Western blot All lanes: ELAVL4 antibody at 2microg/ml Lane 1: MCF-7 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 41, 43 kDa Observed band size: 42 kDa

ELAVL4 Antibody

CSB-PA007595LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetELAVL4
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Overview

  • Supplier
    Cusabio
  • Product Name
    ELAVL4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1996
  • Target name
    ELAVL4
  • Target description
    ELAV like RNA binding protein 4
  • Target synonyms
    HUD, PNEM, ELAV-like protein 4, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D), ELAV like neuron-specific RNA binding protein 4, Hu antigen D, paraneoplastic encephalomyelitis antigen HuD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26378
  • Protein Name
    ELAV-like protein 4
  • Scientific Description
    May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: U87-MG whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL4 antigen affinity purified polyclonal antibody (Catalog # PB9698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL4 at approximately 42KD. The expected band size for ELAVL4 is at 42KD.
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