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ProductsBack/ELAVL4 / HuD Antibody (HRP)

ELAVL4 / HuD Antibody (HRP)

LS-C375640
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetELAVL4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ELAVL4 / HuD Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID1996
  • Target name
    ELAVL4
  • Target description
    ELAV like RNA binding protein 4
  • Target synonyms
    HUD, PNEM, ELAV-like protein 4, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D), ELAV like neuron-specific RNA binding protein 4, Hu antigen D, paraneoplastic encephalomyelitis antigen HuD
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: U87-MG whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL4 antigen affinity purified polyclonal antibody (Catalog # PB9698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL4 at approximately 42KD. The expected band size for ELAVL4 is at 42KD.
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