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ProductsBack/ELF1 Antibody - N-terminal region (ARP31085_P050)

ELF1 Antibody - N-terminal region (ARP31085_P050)

ARP31085_P050
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat
TargetELF1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ELF1 Antibody - N-terminal region (ARP31085_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID1997
  • Target name
    ELF1
  • Target description
    E74 like ETS transcription factor 1
  • Target synonyms
    EFTUD1, RIA1, ETS-related transcription factor Elf-1, E74-like factor 1 (ets domain transcription factor)
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of E74 like factor 1 using anti-E74 like factor 1 antibody (A03187-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: Human A431 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E74 like factor 1 antigen affinity purified polyclonal antibody (Catalog # A03187-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E74 like factor 1 at approximately 75KD. The expected band size for E74 like factor 1 is at 67KD.
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