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Elongation Factor Ts Antibody (YA2524, Rabbit)

HY-P82779
MedChem Express
TargetTSFM
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Elongation Factor Ts Antibody (YA2524, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10102
  • Target name
    TSFM
  • Target description
    Ts translation elongation factor, mitochondrial
  • Target synonyms
    EFTS, EFTSMT, elongation factor Ts, mitochondrial, mitochondrial elongation factor Ts
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Elongation Factor Ts Antibody (YA2524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Elongation Factor Ts.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EF-Ts/TSFM using anti-EF-Ts/TSFM antibody (A08656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EF-Ts/TSFM antigen affinity purified polyclonal antibody (Catalog # A08656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EF-Ts/TSFM at approximately 35 kDa. The expected band size for EF-Ts/TSFM is at 35 kDa.
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