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TSFM Antibody

LS-C834980
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTSFM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TSFM Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.98 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10102
  • Target name
    TSFM
  • Target description
    Ts translation elongation factor, mitochondrial
  • Target synonyms
    EFTS, EFTSMT, elongation factor Ts, mitochondrial, mitochondrial elongation factor Ts
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EF-Ts/TSFM using anti-EF-Ts/TSFM antibody (A08656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EF-Ts/TSFM antigen affinity purified polyclonal antibody (Catalog # A08656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EF-Ts/TSFM at approximately 35 kDa. The expected band size for EF-Ts/TSFM is at 35 kDa.
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