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ProductsBack/EN1 / Engrailed Antibody

EN1 / Engrailed Antibody

LS-C498771
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityChicken
TargetEN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EN1 / Engrailed Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2019
  • Target name
    EN1
  • Target description
    engrailed homeobox 1
  • Target synonyms
    ENDOVESLB, homeobox protein engrailed-1, engrailed homolog 1, homeobox protein en-1, hu-En-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Chicken
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EN1 using anti-EN1 antibody (M05395). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EN1 antigen affinity purified monoclonal antibody (Catalog # M05395) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EN1 at approximately 55 kDa. The expected band size for EN1 is at 40 kDa.
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