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ProductsBack/Engrailed 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Engrailed 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

BS-11744R-BF350
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetEN1
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Overview

  • Supplier
    Bioss
  • Product Name
    Engrailed 1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Other Conjugate
  • Gene ID2019
  • Target name
    EN1
  • Target description
    engrailed homeobox 1
  • Target synonyms
    ENDOVESLB, homeobox protein engrailed-1, engrailed homolog 1, homeobox protein en-1, hu-En-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05925
  • Protein Name
    Homeobox protein engrailed-1
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of EN1 using anti-EN1 antibody (M05395). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EN1 antigen affinity purified monoclonal antibody (Catalog # M05395) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EN1 at approximately 55 kDa. The expected band size for EN1 is at 40 kDa.
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