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ProductsBack/Endoplasmic Reticulum Resident Protein 57 (ERp57) Polyclonal Antibody

Endoplasmic Reticulum Resident Protein 57 (ERp57) Polyclonal Antibody

CAU25815
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPDIA3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Endoplasmic Reticulum Resident Protein 57 (ERp57) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.45 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2923
  • Target name
    PDIA3
  • Target description
    protein disulfide isomerase family A member 3
  • Target synonyms
    58 kDa glucose-regulated protein; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER protein 57; ER protein 60; ER60; ERp57; ERp60; ERp61; glucose regulated protein, 58kDa; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; phospholipase C-alpha; PI-PLC; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3
  • Host
    Rabbit
  • Protein IDP30101
  • Protein Name
    Protein disulfide-isomerase A3
  • Scientific Description
    The Endoplasmic Reticulum Resident Protein 57 (ERp57) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERp57 using anti-ERp57 antibody (PB9772). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Mouse Liver Tissue Lysate, Lane 3: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp57 antigen affinity purified polyclonal antibody (Catalog # PB9772) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp57 at approximately 57KD. The expected band size for ERp57 is at 57KD.
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