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ENO2 Polyclonal Antibody

CAC14115
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetENO2
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Overview

  • Supplier
    Biomatik
  • Product Name
    ENO2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2026
  • Target name
    ENO2
  • Target description
    enolase 2
  • Target synonyms
    2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; gamma-enolase; HEL-S-279; neural enolase; neuron specific gamma enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09104
  • Protein Name
    Gamma-enolase
  • Scientific Description
    The ENO2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSE using anti-NSE antibody (A02930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysate, Lane 2: human U20S whole cell lysate, Lane 3: human A431 whole cell lysate, Lane 4: human HepG2 whole cell lysate, Lane 5: human A549 whole cell lysate, Lane 6: human SHG-44 whole cell lysate, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSE antigen affinity purified polyclonal antibody (Catalog # A02930) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSE at approximately 47KD. The expected band size for NSE is at 47KD.
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