Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101553/GTX101553_44279_20210423_WB_w_23060100_593.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101553/GTX101553_39721_20170310_IHC-Fr_R_w_23060100_807.webp "NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101553/GTX101553_44279_20210416_WB_w_23060100_343.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
NSE antibody [N1C1]
GTX101553
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetENO2
Overview
- SupplierGeneTex
- Product NameNSE antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.13 mg/ml
- ConjugateUnconjugated
- Gene ID2026
- Target nameENO2
- Target descriptionenolase 2
- Target synonymsHEL-S-279, NSE, gamma-enolase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, enolase 2 (gamma, neuronal), epididymis secretory protein Li 279, neural enolase, neuron specific gamma enolase, neuron-specific enolase, neuronal enriched enolase, neurone-specific enolase
- HostRabbit
- IsotypeIgG
- Protein IDP09104
- Protein NameGamma-enolase
- Scientific DescriptionThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ugun-Klusek A, Tatham MH, Elkharaz J, et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway. Cell Death Dis. 2017,8(1):e2531. doi: 10.1038/cddis.2016.443Read this paper
- Lin TP, Chang YT, Lee SY, et al. REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling. Oncotarget. 2016,7(18):26137-51. doi: 10.18632/oncotarget.8433Read this paper
Datasheet
Related products
Product group Antibodies
Anti-NSE [E1-7E2]Ab03096-1.1
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetENO2
- SizePrice
Product group Antibodies
ApplicationsELISA, ImmunoHistoChemistry
- SizePrice
Product group Antibodies
Anti-ENO2 Antibody144-03118
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetENO2
- SizePrice
Product group Antibodies
ENO2 Polyclonal AntibodyCAC14115
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetENO2
- SizePrice
Product group Antibodies
References
NSE (15E2) Monoclonal AntibodyBSM-33072M
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetENO2
- SizePrice
Product group Antibodies
ENO2 Monoclonal AntibodyCSB-MA000224
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetENO2
- SizePrice
Product group Antibodies
Anti-NSE AntibodyA104334
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
- SizePrice
Product group Antibodies
ENO2 / NSE AntibodyLS-C834942
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetENO2
- SizePrice
Product group Antibodies
NSE antibody (Ready-to-use)GTX15488
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetENO2
- SizePrice