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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS antibody (GTX129113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

eNOS antibody

GTX129113
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNOS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    eNOS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4846
  • Target name
    NOS3
  • Target description
    nitric oxide synthase 3
  • Target synonyms
    ECNOS, eNOS, nitric oxide synthase 3, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, nitric oxide synthase 3 (endothelial cell)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29474
  • Protein Name
    Nitric oxide synthase 3
  • Scientific Description
    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Jiang YH, Kuo HC. Urothelial Barrier Deficits, Suburothelial Inflammation and Altered Sensory Protein Expression in Detrusor Underactivity. J Urol. 2017,197(1):197-203. doi: 10.1016/j.juro.2016.07.071
    Read this paper

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Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: human K562 whole cell lysates Lane 3: human HepG2 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat kidney tissue lysates Lane 6: mouse kidne tissue lysates Lane 7: mouse small intestine tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS3 at approximately 133KD. The expected band size for NOS3 is at 133KD.
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